| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7694539 | Current Opinion in Chemical Biology | 2015 | 6 Pages |
Abstract
- Protein-dye complexes that activate fluorescence allow no-wash protein labeling.
- Quencher removal, environmental change and direct binding can activate fluorescence.
- Modifications of the chromophore and linker can influence fluorogen performance.
- Pulse-chase and order-of-addition experiments can reveal dynamic cellular changes.
- High-quality fluorogen imaging in organisms will reveal new biological processes.
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Marcel P Bruchez,