| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7694540 | Current Opinion in Chemical Biology | 2015 | 7 Pages |
Abstract
- Two-photon imaging of biosensors in intact tissue is challenging to calibrate.
- Ratiometric or fluorescence lifetime imaging can provide the needed normalization.
- Calibrated ratiometric imaging requires depth and power correction.
- Fluorescence lifetime imaging provides a calibrated readout of sensor occupancy.
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Gary Yellen, Rebecca Mongeon,