Heavy enzymes - experimental and computational insights in enzyme dynamics

- Kinetic studies on isotopically substituted enzymes are a useful tool to evaluate the impact of protein motions in the chemical step.
- Recent experimental and theoretical works on enzyme kinetic isotope effects are reviewed.
- The observed enzymatic kinetic isotope effects can be interpreted using Transition State Theory.
- Protein motions of different time-scales can participate in the reaction coordinate although the use of the term 'dynamical effects' can be misleading.