Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7702501 | Ultrasonics Sonochemistry | 2018 | 30 Pages |
Abstract
Low energy ultrasound irradiation was used to enhance co-production of enzymes uricase and alkaline protease using Bacillus licheniformis NRRL 14209. Production of uricase and alkaline protease was evaluated for different ultrasound parameters such as ultrasound power, time of irradiation, duty cycle and growth stage of organisms at which irradiation is carried out. Maximum uricase production of 0.825â¯U/mL and alkaline protease of 0.646â¯U/mL have been obtained when fermentation broth was irradiated at 6â¯h of growth stage with 60â¯W power for 15â¯min of duration having 40% of duty cycle. The enzyme yield was found to be enhanced by a factor of 1.9-3.8 and 1.2-2.2 for uricase and alkaline protease respectively. Nevertheless, intracellular uricase was also observed in a fermentation broth after ultrasonic process intensification. The results indicate the effectiveness of low frequency ultrasound in improving enzyme yields with a vision of commercial applicability of the process.
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Authors
Shweta V. Pawar, Virendra K. Rathod,