Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7702726 | Ultrasonics Sonochemistry | 2018 | 33 Pages |
Abstract
Galactooligosaccharides (GOS), recognised prebiotic, can be industrially produced from lactose and commercial β-galactosidase (β-gal) from Kluyveromyces lactis. Residual lactose and glucose limit GOS applications. To handle this problem, a multienzymatic system, with β-gal and glucose oxidase (Gox), was proposed to reduce glucose content in reaction media through its oxidation to gluconic acid (GA). Besides, ultrasound (US) probe effect over the multienzymatic system to produce GOS and GA has been evaluated. A production around 40% of GOS was found in all treatments after the first hour of reaction. However, glucose consumption and GA production was significantly higher (Pâ¯<â¯0.05) for sequential reaction assisted by US, obtaining the best production of GOS (49%) and GA (28%) after 2â¯h of reaction. The conformational and residual activity changes of enzymes under US conditions were also evaluated, Gox being positively affected whereas in β-gal hardly any change was found.
Related Topics
Physical Sciences and Engineering
Chemistry
Chemistry (General)
Authors
Fabián Rico-RodrÃguez, Juan Carlos Serrato, Antonia Montilla, Mar Villamiel,