| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7754504 | Journal of Inorganic Biochemistry | 2016 | 9 Pages |
Abstract
Metallo-β-lactamases (MBLs) catalyse the hydrolysis of almost all β-lactam antibiotics and are a growing clinical problem. It is proposed that MBLs employ one or two zinc ion cofactors. We report that MBLs can employ ferrous iron, but with altered kinetic and inhibition profiles compared to the zinc enzymes.292
Keywords
Zinc hydrolaseVIM-2SBLPCEETHE1IPTGmetalloenzymeIC50CPSFNDM-1ROOPMSFESI MSMBLSNMβ-Lactam antibioticsEDTAEthylenediaminetetraacetic acidSDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresisisopropyl β-D-1-thiogalactopyranosideelectrospray ionisation mass spectrometryphenylmethylsulfonyl fluorideMetallo-β-lactamaseAntibiotic resistancehalf maximal inhibitory concentrationCarbapenem
Related Topics
Physical Sciences and Engineering
Chemistry
Inorganic Chemistry
Authors
Samuel T. Cahill, Hanna Tarhonskaya, Anna M. Rydzik, Emily Flashman, Michael A. McDonough, Christopher J. Schofield, Jürgen Brem,