Chemical constituents isolated from the rhizomes of Helleborus odorus subsp. cyclophyllus (Ranunculaceae)

Chemical investigation of butanol extract from the rhizomes of Helleborus odorus subsp. cyclophyllus led to the isolation of nine natural products, which are identified, on the basis of MS and NMR spectra as 2-hydroxymethyl-D-ribono-γ-lactone (1), uridine (2), 2-(3,4-dihydroxy)-phenyl-ethyl-β-D-glucopyranoside (3), the bufadienolides deglucohellebrin (4) and hellebrin (5), the furostanols caucasicoside A (6) and helleboroside B (7) and ecdysones 20-hydroxyecdysone (8) and polypodine B (9). This is the first report on the occurrence of compounds (1)-(3) in the genus Helleborus.