Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7768152 | Biochemical Systematics and Ecology | 2015 | 6 Pages |
Abstract
The 14-3-3 proteins are a large family of approximately 30Â kDa acidic proteins and acting in the regulation of many biological processes. In this study, a 14-3-3 zeta (Pi14-3-3z) gene from the Indian meal moth, Plodia interpunctella (Lepidoptera, Pyralidae) was isolated and characterized. The full-length cDNA of Pi14-3-3z is 1382Â bp, including a 5'-untranslated region (UTR) of 141Â bp, 3â²-UTR of 497Â bp and an open reading frame (ORF) of 744Â bp encoding a polypeptide of 247 amino acids which contains a 14-3-3 homologues domain (PF00244). The deduced Pi14-3-3z protein sequence has 81%-100% identity with the homologues in comparison to with other individuals. qPCR analysis revealed that Pi14-3-3z was expressed at the four developmental stages and in all tissues tested. Based on the amino acid of 14-3-3z, phylogenetic analysis demonstrated a similar topology with the traditional classification, suggesting 14-3-3z protein has the potential value in phylogenetic inference.
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Authors
Qiu-Ning Liu, Dan-Dan Bian, Bao-Ming Ge, Chun-Lin Zhou, Bo-Ping Tang,