Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7769258 | Biochemical Systematics and Ecology | 2013 | 6 Pages |
Abstract
Salvia miltiorrhiza, known as Dan-shen in China, is a well-known and important medicinal herb in Asia. Its root and rhizome has been used to treat cardiovascular diseases in China for centuries. Recently, it had been widely cultivated in China due to a rapid decline in wild resources. However, the germplasm resources of different cultivated regions are extremely variable. Here, new simple sequence repeats (SSRs) from expressed sequence tags (ESTs) were mined to analyze the frequency and distribution of SSRs, assess the validity of developed EST-SSR marker and identify new EST-SSR marker for the preparatory investigation of the population structure in cultivated S. miltiorrhiza. This study showed that 5160 unique ESTs were accumulated from database, 760 SSRs combined with 41 types of SSR motifs were mined. High polymorphism and transferability were presented in newly developed EST-SSR marker. It also showed that cultivated S. miltiorrhiza were mainly divided into three large groups. The results suggested that S. miltiorrhiza may has high abundance of SSRs in transcribed regions of genome and the developed EST-SSR marker may be useful for analysis of genetic diversity and population structure.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Guojie Xu, Chunsheng Liu, Luqi Huang, Xueyong Wang, Yuanyuan Zhang, Siqi Liu, Caili Liao, Qingjun Yuan, Xiaoli Zhou,