Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7780997 | Carbohydrate Polymers | 2018 | 8 Pages |
Abstract
Arabinogalactans are a source of dietary fiber with health benefits. In this work, two arabinogalactans assigned as AGW and AGS were isolated from Larix principis-rupprechtii, and characterized by gel permeation chromatography (GPC), monosaccharide analysis, methylation analysis and NMR spectroscopy analysis. The average molecular weights of AGW and AGS were 1.53âÃâ104 and 1.84âÃâ104 Da, respectively. Methylation analysis and NMR spectra suggested that AGW and AGS have a 1,3-linked Galp backbone, branched at C-6 with 1,6-linked Galp side residues. The Ara residues were substituted at C-6 of 1,6-linked Galp consisting of α-L-Araf-(1â3)-α-L-Araf-(1â6)-β-D-Galp-(1â and β-L-Arap-(1â6)-β-D-Galp-(1â. Significantly, AGS (0.74%) was shown to contain 25 times more uronic acid than AGW (0.03%), which demonstrated a polyelectrolyte effect. Application of these two polysaccharides to macrophage RAW 264.7 cells was shown to increase nitric oxide (NO) production. These results provide a basis for studying the relationship between the structure and biological activity of arabinogalactans.
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Authors
Shuo Tang, Meiyun Jiang, Caoxing Huang, Chenhuan Lai, Yimin Fan, Qiang Yong,