Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7781507 | Carbohydrate Polymers | 2018 | 37 Pages |
Abstract
A novel heteropolysaccharide from Grifola frondosa named GFP-W has been isolated and purified by DEAE Sephadex A-52 chromatography. Gas chromatography, fourier transform infrared spectroscopy, one-dimensional (1H- and 13C-) and two-dimensional (1H-1H COSY, 1H-13C HSQC, and 1H-13C HMBC) nuclear magnetic resonance spectroscopy were used to characterize its structure. The average molecular weight of GFP-W was 66.1â¯kDa. GFP-W mainly contained four kinds of linkage type units as β-D-GlcpAâ, 1,2,6-α-Gal, â2)-α-Manpâ, and â3)-α-L-Fucp-(1â. It could significantly increase the uptake of glucose in dexamethasone induced insulin resistant HepG2 cells by improving the mRNA and protein expression of insulin receptor substrate 1, phosphatidylinositol-3-kinase, and glucose transporter 4 upregulation and c-Jun N-terminal Kinase 1 downregulation. Moreover, antidiabetic activities of GFP-W were associated with significant changes in the extent of protein lysine acetylation, crotonylation, and succinylation levels. Our results provide a new hypoglycemic therapeutic role and an in-depth analysis on molecular mechanisms upon polysaccharides from G. frondosa.
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Authors
Yuqing Chen, Yuanyuan Liu, Md. Moklesur Rahman Sarker, Xin Yan, Chengfeng Yang, Lina Zhao, Xucong Lv, Bin Liu, Chao Zhao,