Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7781620 | Carbohydrate Polymers | 2018 | 27 Pages |
Abstract
A new water-soluble polysaccharide (FVP1) was extracted from Flammulina velutipes by traditional method “water extraction and alcohol precipitation” and purified by column chromatography. Physicochemical characterization showed that FVP1 was a homogeneous polysaccharide with a relative molecular weight of 54.78â¯kDa. It is composed of mannose (7.74%), glucose (70.41%), and galactose (16.38%). FVP1 (1000â¯mg/mL) possessed significant immune activity by increasing the secretion of nitric oxide (NO), tumour necrosis factor-α (TNF-α) (3183â¯Â±â¯133.84â¯pg/mL), interleukin (IL)-6 (1133.21â¯Â±â¯39.05â¯pg/mL), and IL-12 (579.96â¯Â±â¯74.53â¯pg/mL) in macrophages. Furthermore, FVP1 showed significant hepatitis B surface antibody (anti-HBV) activity through reducing the expression of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA replication. These results suggest a novel role for FVP1 to be applied as an immunomodulators in dietary supplements to prevent HBV infection.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Tiantian Zhang, Jufeng Ye, Changhu Xue, Yuming Wang, Wenzhen Liao, Limei Mao, Miaomiao Yuan, Sen Lian,