Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7781997 | Carbohydrate Polymers | 2018 | 37 Pages |
Abstract
Native (F2) and carboxyl-reduced (R) ulvans from Ulva fasciata were sequentially oxidized with periodate-chlorite affording the polycarboxyl ulvans C1, C2 and C3 (1.20, 1.41 and 1.81â¯mmolâ¯gâ1 of COOH, respectively; 19.7, 21.3 and 21.0% of NaSO3, respectively) and R-C3 (1.86â¯mmolâ¯gâ1 of COOH; NaSO3â¯=â¯22.7%), respectively. APTT assay (polysaccharide fractions at 150â¯Î¼gâ¯mLâ1) showed clotting time of 45.6â¯s for F2 fraction. For polycarboxyl ulvans C1, C2, C3 and R-C3 the clotting times were 101.0, 122.2, 222.0 and 227.0â¯s, respectively. Comparison of the APTT assay results using ulvans chemically modified by carboxyl-reduction, desulfation, periodate oxidation and/or chlorite oxidation showed the anticoagulant activity of polycarboxyl ulvans is dependent of the sulfate groups present in the native polymer. In addition, the increase of the anticoagulant activity was accompanied by the increasing of the carboxyl groups and the content of this acidic substituent seems to be more important than its positioning.
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Authors
Mariana M. de Carvalho, Rilton A. de Freitas, Diogo R.B. Ducatti, Luciana G. Ferreira, Alan G. Gonçalves, Franciely G. Colodi, Ester Mazepa, Estela M. Aranha, Miguel D. Noseda, Maria Eugênia R. Duarte,