Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7782886 | Carbohydrate Polymers | 2018 | 7 Pages |
Abstract
An aqueous extract containing polysaccharides was obtained from the giant mushroom Macrocybe titans, and it was purified by amylase treatment, freeze-thawing process and dialysis. The purified fraction (ESP) was analyzed by HPSEC and GC-MS which showed a homogenous polysaccharide with Mw 14.2â¯Ãâ¯103â¯g/mol composed by galactose and fucose. NMR and methylation analysis of ESP confirmed the presence of a fucogalactan with a (1â¯ââ¯6)-linked α-d-Galp main chain partially substituted at O-2 by non reducing end units of α-l-Fucp residues in the side chain. Its biological activity was evaluated against murine melanoma cells B16-F10. The fucogalactan did not alter the viability, proliferative capacity and morphology of cells. However, this polysaccharide was able to reduce the cell migration in vitro at 40% (100â¯Î¼g/mL) and 33% (250â¯Î¼g/mL). The results obtained showed that Macrocybe titans fucogalactan is a promising agent capable of altering melanoma cell migration without decrease the cell viability.
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Authors
Shayane da Silva Milhorini, Fhernanda Ribeiro Smiderle, Stellee Marcela Petris Biscaia, Fabio Rogerio Rosado, Edvaldo S. Trindade, Marcello Iacomini,