Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7782936 | Carbohydrate Polymers | 2018 | 31 Pages |
Abstract
Chondroitin sulfate (CS) was regio-specifically modified to an unsaturated derivative (ÎCS) with a double bond in positions 4 and 5 of N-acetyl-d-galactosamine. The structure of ÎCS was elucidated in detail by two dimensional nuclear magnetic resonance, ultraviolet spectroscopy and mass spectrometry. The introduction of a nucleophilic CC double bond into a polymer backbone had no influence on biocompatibility of CS, which was demonstrated by MTT live-dead assay and enzymatic degradation in vitro. On the other hand the chemical modification significantly enhanced the reactivity of ÎCS towards numerous oxidizing agents, which might be promising for a variety of biomedical and cosmetic applications.
Keywords
JHHGAGHSQCGalNAcPBSnuclear magnetic resonanceLC–MSm/zN-Acetyl-D-GalactosamineUV/Visw/vUltraviolet and visible spectroscopyReductionOxidationNMRChemical shiftDiffusion Ordered SpectroscopYDegree of substitutiontwo dimensionalliquid chromatography–mass spectrometrycorrelation spectroscopyDOSYPhosphate buffered salineAntioxidant activityCosmeticsmass to charge ratioweight per volumeMolecular weightCOSYhigh-performance liquid chromatographyHPLCChondroitin sulfateheteronuclear single quantum coherence
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
TomáÅ¡ Bobula, Radovan Buffa, Martina Hermannová, Hana Vágnerová, Iva DoleÄková, VladimÃr Velebný,