Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7782981 | Carbohydrate Polymers | 2018 | 25 Pages |
Abstract
This study investigated the chemical characterization and antitumor effects of a polysaccharide from ramulus mori. A water-soluble polysaccharide, RMP1 with the estimated molecular weight of 137â¯kDa, was isolated and purified from ramulus mori through gel permeation chromatography. RMP1 is mainly composed of arabinose, xylose, glucose, galactose and rhamnose in a ratio of 0.56:0.37:0.17:1.00:0.08. Methylation and NMR analysis revealed that RMP1 had a backbone composed of 1,6-β-d-Galp, 1,3,6-β-d-Galp and 1,3-β-d-Galp residues, two main branches of 1,2-α-l-Araf, 1,3,6-β-d-Galp and 1,4-β-d-Xylp; it also had α-l-Araf and β-d-Glcp as terminals. In the MTT assay, RMP1 showed significant anticancer effects against the SGC-7901 and HeLa cells. In addition, no cytotoxicity was observed on the HEK-293 and RAW 264.7 cells. Flow cytometry showed that RMP1 exerted a stimulatory effect on the SGC-7901 cells apoptosis and induced the cell cycle arrest at the S phases. These findings suggest that RMP1 may serve as a potential novel antitumor agent.
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Authors
Yajie Chen, Xue Jiang, Hongqing Xie, Xiaotong Li, Liangen Shi,