Hyaluronate dots for highly efficient photodynamic therapy

Nanoscale particles, such as quantum dots and carbon dots, are important materials for use in sensing and treating irregular biological events, but their versatility for biomedical applications are usually limited by their undesirable properties such toxicity and non-degradability. Here, we report biofunctional hyaluronic acid (HA) dots containing biodegradable/biocompatible HA. HA dots were prepared by conjugating multiple HA molecules to C60 (used as a base dot) without any hydrothermal treatment. The hydroxyl groups of HA completely linked to all π-π carbon bonds in C60. The chemically synthesized HA dots (Mn = 16.1 kDa) were 2 nm in diameter, soluble in aqueous solution, and possessed multiple functional (carboxyl) groups. The HA dots were biofunctional, enabling highly efficient binding to CD44 receptors overexpressed on in vitro/in vivo tumors. With light illumination, we demonstrated that the HA dots bearing a photosensitizing model drug (chlorin e6: Ce6) resulted in a significant enhancement in in vitro/in vivo tumor cell ablation. We believe that this approach offers a new strategy to create biopolymer dots.