Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7785288 | Carbohydrate Polymers | 2016 | 7 Pages |
Abstract
In the present study, the crude polysaccharide was extracted from Fagopyrum tartaricum and purified by Sephadex G-25 and G-75 column to produce a polysaccharide fraction termed TBP-II. Its average molecular weight was 26 kDa. The structural characterization of TBP-II was investigated by gas chromatography, periodate oxidation-Smith degradation, Methylation and NMR. Congo red was applied to explore its advanced structures. The results revealed that chemical composition and structural characteristic of TBP-II was mainly consisted of galactose, arabinose, xylose and glucose with a molar ratio of 0.7:1:6.3:74.2. The backbone of TBP-II was composed of (1 â 4)-linked α-d-glucopyranosyl (Glcp), while the branches comprised of (1 â 3)-linked α-d-glucopyranosyl (Glcp), (1 â 6)-linked α-d-galactopyranosyl (Galp) and (1 â 2,4)-linked α-d-rhamnopyranosyl (Rhap). The structure of TBP-II was 1,3 and 1,6-branched-galactorhamnoglucan that had a linear backbone of (1 â 4)-linked α-d-glucopyranose (Glcp). Using Congo red assay showed that it was absent of triple helix structure. The α-d-glucosidase inhibitory activity of TBP-II was determined using acarbose as positive control. The result showed that the inhibition rate depended on the concentration of polysaccharides.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Xiao-Ting Wang, Zhen-Yuan Zhu, Liang Zhao, Hui-Qing Sun, Meng Meng, Jin-Yu Zhang, Yong-min Zhang,