Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7785732 | Carbohydrate Polymers | 2016 | 9 Pages |
Abstract
Bacterial exopolysaccharides (EPSs) are promising sustainable alternatives to synthetic polymers. Here we describe the production and characterization of different EPSs produced by the recently isolated Paenibacillus sp. 2H2. A final EPS titer of 4.54 g Lâ1 was recovered after a 17-h fermentation, corresponding to a volumetric productivity of 0.27 g Lâ1 hâ1. Remarkably, supplying the fermentation with specific carbon and nitrogen sources could be exploited for the production of different polymers. A pure heteropolysaccharide composed of glucose, mannose, galactose, and glucuronic acid (3.5:2:1:0.1) was obtained when using glucose/glycerol and peptone as substrates. A pure levan-type polymer or mixture of both polymers was observed with sucrose and NaNO3 or peptone. To our knowledge, this is the first report that nutrients, particularly nitrogen sources, can be used to fine-tune EPS production in Paenibacillaceae. Rheological characterization of the heteropolysaccharide revealed impressive thickening properties, suggesting its potential application in commodity materials.
Related Topics
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Authors
Marius Rütering, Jochen Schmid, Broder Rühmann, Martin Schilling, Volker Sieber,