Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7786083 | Carbohydrate Polymers | 2016 | 34 Pages |
Abstract
Dermatan sulfate (DS) is one of the hardest impurities to remove from heparin products due to their high structural similarity. The development of a sensitive and feasible method for quantitative detection of DS in heparin is essential to ensure the clinical safety of heparin pharmaceuticals. In the current study, based on the substrate specificity of chondroitin B lyase, ultraviolet spectrophotometric and strong anion-exchange high-performance liquid chromatographic methods were established for detection of DS in heparin. The former method facilitated analysis in heparin with DS concentrations greater than 0.1 mg mLâ1 at 232 nm, with good linearity, precision and recovery. The latter method allowed sensitive and accurate detection of DS at concentrations lower than 0.1 mg mLâ1, exhibiting good linearity, precision and recovery. The linear range of DS detection using the latter method was between 0.01 and 0.5 mg mLâ1.
Keywords
SAXUSPGAGisopropyl-β-d-1-thiogalactopyranosideIPTGMBPLOQIdoARSDOSCsE.coliUltravioletUltraviolet spectrophotometryrelative standard deviationUnited States Pharmacopeiastrong anion exchangeLOD یا Limit of detectionDermatan sulfateOversulfated chondroitin sulfateEuropean PharmacopoeiaChinese Pharmacopoeialimit of quantificationlimit of detectionHeparan sulfateHeparininternational unitmaltose binding proteinhigh performance liquid chromatographyHPLCChondroitin sulfateGlycosaminoglycan
Related Topics
Physical Sciences and Engineering
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Authors
Jingjun Wu, Yang Ji, Nan Su, Ye Li, Xinxin Liu, Xiang Mei, Qianqian Zhou, Chong Zhang, Xin-hui Xing,