Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7786216 | Carbohydrate Polymers | 2016 | 7 Pages |
Abstract
Cross-linked cellulose microspheres (CL-CMs) were successfully prepared by inverse crosslinking suspension method. NaOH/urea aqueous solution was used as solvent to dissolve cellulose at low temperature. The microspheres presented good spherical shape and monodispersity, which were applied to synthesize endotoxin adsorbent with polymyxin B (PMB) as ligand. The adsorbent showed good adsorption capability on endotoxin in physiologic saline solution and the maximum adsorption capacity was 3605Â EU/g (1EUÂ =Â 100Â pg). It was worth noting that more than 70% of endotoxin could be effectively removed from the human plasma with the initial concentration of endotoxin ranged from 1Â EU/mL to 5Â EU/mL. The dynamic adsorption efficiency of endotoxin was 72.3% at the plasma perfusion rate of 300Â mL/h with the endotoxin concentration of 4Â EU/mL, while the variation of plasma protein before and after adsorption was only 8.9%. It suggests that the PMB immobilized CL-CMs have great potential application in clinical blood purification.
Keywords
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Xiaodong Cao, Biyan Zhu, Xufeng Zhang, Hua Dong,