Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7787126 | Carbohydrate Polymers | 2015 | 7 Pages |
Abstract
Grifola frondosa has long been known and respected as a medically important fungus. This study investigated the characterization, antitumor and immunomodulatory activity of a polysaccharide named GP11 purified from G. frondosa. The results revealed that GP11 was composed of â1)-d-Manp-(6â,â1)-d-Glcp-(4â,â1)-d-Galp-(6âandâ2,3,6)-d-Glcp-(1â, with branches attached at O-2,3 of 1,2,3,6-linked Glcp residues and terminal T-Glcp. GP11 exhibited indirect cytotoxic activity against HepG-2 cells in vitro, and it significantly inhibited the growth of Heps cells in vivo. GP11 increased the relative thymus and spleen weights as well as serum tumor necrosis factor-alpha and interleukin-2 levels. GP11 stimulated tumoricidal activity and the production of nitric oxide (NO), TNF-α and interleukin-1β, and it also stimulated the protein expression of iNOS and mRNA expression of iNOS and TNF-α. TLR-4 is a potential receptor for GP11-mediated macrophage activation. The results suggested that the antitumor activity of GP11 may be due to the improvement of immune functions through the TLR-4-mediated up-regulation of NO and TNF-α.
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Authors
Guang-Hua Mao, Yi Ren, Wei-Wei Feng, Qian Li, Hui-Yu Wu, Dun jin, Ting Zhao, Cai-Quan Xu, Liu-Qing Yang, Xiang-Yang Wu,