Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7787388 | Carbohydrate Polymers | 2015 | 33 Pages |
Abstract
Sea cucumber fucoidan is a major bioactive component of sea cucumber. The structures of fucoidans have significant influences on their biological activities. The present study clarified the delicate structure of a fucoidan from Pearsonothuria graeffei. Fucoidan was obtained after papain digestion and purified by ion chromatography. The carbohydrate sequence of fucoidan was firstly determined by negative-ion electrospray tandem mass spectrometry (ES-MS) with collision-induced dissociation of the oligosaccharide fragments, which were obtained by mild acid hydrolysis, and completed by NMR for assignment of the anomeric conformation. It was unambiguously identified as a tetrasaccharide repeating unit with a backbone of [â3Fuc (2S, 4S) α1 â 3Fucα1 â 3Fuc (4S) α1 â3 Fuc#7Ã10#]n. The glycosidic bonds between the non-sulfated and 2,4-O-disulfated fucose residues were selectively cleaved, and highly ordered oligosaccharide fragments with a tetrasaccharide repeating unit were obtained. The highly 4-O- and 2, 4-di-O-sulfated polysaccharide deserves further developments for Pharmacia use.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Yaqin Hu, Shan Li, Junhui Li, Xingqian Ye, Tian Ding, Donghong Liu, Jianchu Chen, Zhiwei Ge, Shiguo Chen,