Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7787840 | Carbohydrate Polymers | 2015 | 11 Pages |
Abstract
Acemannan, an acetylated polymannose from Aloe vera, induces tissue repair. We investigated the role of acemannan's acetyl-groups on its physical and biological properties. Deacetylated acemannan (DeAcAM) was prepared and characterized. The physical properties and microscopic structure of DeAcAM were evaluated using water solubility, contact angle, X-ray diffraction, and scanning-electron microscopy. The activity of DeAcAM on cell proliferation and gene expression were assessed. Acemannan and DeAcAM structures were simulated and the acemannan tetramer diad and its completely deacetylated structure were also determined. Increased acemannan deacetylation reduced its water solubility and hydrophilicity. Complete deacetylation altered acemannan's conformation to a partial crystal structure. The bioactivity of acemannan was reduced corresponding to its deacetylation. Acemannan induced cell proliferation, and VEGF and Collagen I expression; however, 100% DeAcAM did not. The simulated structures of the acemannan diad and the completely deacetylated diad were different. We conclude acetyl-groups affect acemannan's structure and physical/biological properties.
Keywords
AcemannanSemi-empirical AM1 methodMDAHGFsDDATDAddH2OPBSMWCOSECDMEM3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideDMSOMTTdouble-distilled waterAloe veraSize-exclusion chromatographyDegree of deacetylationDeacetylationDimethyl sulfoxideVascular endothelial growth factorVascular Endothelial Growth Factor (VEGF)Phosphate buffered salineBioactivityHuman gingival fibroblastsMolecular weight cutoffDulbecco’s modified eagle’s medium
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Jaroenporn Chokboribal, Wanpen Tachaboonyakiat, Polkit Sangvanich, Vithaya Ruangpornvisuti, Suwimon Jettanacheawchankit, Pasutha Thunyakitpisal,