Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7788583 | Carbohydrate Polymers | 2015 | 38 Pages |
Abstract
An exopolysaccharide (EPS)-producing strain YW11 isolated from Tibet Kefir was identified as Lactobacillus plantarum, and the strain was shown to produce 90 mg Lâ1 of EPS when grown in a semi-defined medium. The molecular mass of the EPS was 1.1 Ã 105 Da. The EPS was composed of glucose and galactose in a molar ratio of 2.71:1, with possible presence of N-acetylated sugar residues in the polysaccharide as confirmed by NMR spectroscopy. Rheological studies showed that the EPS had higher viscosity in skim milk, at lower temperature, or at acidic pH. The viscous nature of the EPS was confirmed by observation with scanning electron microscopy that demonstrated a highly branched and porous structure of the polysaccharide. The atomic force microscopy of the EPS further revealed presence of many spherical lumps, facilitating binding with water in aqueous solution. The EPS had a higher degradation temperature (287.7 °C), suggesting high thermal stability of the EPS.
Keywords
Sodium nitrate (PubChem CID: 24268)Rhamnose (PubChem CID: 25310)Trifluoroacetic acid (PubChem CID: 6422)Sulfuric acid (PubChem CID: 1118)ExopolysaccharideRheologyFructose (PubChem CID: 5984)Phenol (PubChem CID: 996)Lactobacillus plantarumStructural characterizationSodium chloride (PubChem CID: 5234)Glucose (PubChem CID: 79025)Glycerol (PubChem CID: 753)
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Ji Wang, Xiao Zhao, Zheng Tian, Yawei Yang, Zhennai Yang,