Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7788658 | Carbohydrate Polymers | 2015 | 35 Pages |
Abstract
Hyaluronan (HA) was chemically modified to a photocurable derivative by the acylation with a mixed anhydride of trans-cinnamic acid and characterized by UV, IR and 2D NMR spectroscopy. Photocurable HA was processed to a microfibrous structure by wet-spinning technology. Water solubility of otherwise water-soluble HA microfibres was reduced significantly by the solid-state photocrosslinking. We also investigated that the nature of polymer structure had a great impact to a final crosslink ratio. The analysis of the mechanical properties showed higher ultimate tensile strength and increased rigidity of the photocrosslinked fibres in comparison to the untreated ones. Moreover all tested materials are regarded as biocompatible according to the tests of cell viability, phototoxicity and enzymatic degradability, which make them suitable candidates for numerous biomedical applications.
Keywords
3T34-dimethylaminopyridineDMAPHSQCδDIPATHFλmaxBTHSEC-MALLSλemFBSSDSDMEMInteraction constantOxolaneΔxPBSstandard fibroblast cell linenuclear magnetic resonance3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromideDulbecco's modified Eagle MediumMTTUV/Visv/vCinnamic acidUltraviolet and visible spectroscopyFibresisopropyl alcoholYieldtriethylamineNMRChemical shiftDiffusion Ordered SpectroscopYvolume per volumeDegree of substitutionIrradiation dosesodium lauryl sulfatefetal bovine serumFTIRFourier transform infrared spectroscopycorrelation spectroscopyswelling capacityPhotocrosslinkingDOSYPhosphate buffered salineSEMWet-spinningScanning electron microscopyHYAHyaluronanbovine testicular hyaluronidaseMolecular weightTEACOSYheteronuclear single quantum coherence
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
TomáÅ¡ Bobula, JiÅà BÄÅ¥ák, Radovan Buffa, Martina Moravcová, Pavel Klein, OndÅej Židek, Veronika Chadimová, Robert PospÃÅ¡il, VladimÃr Velebný,