Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7788893 | Carbohydrate Polymers | 2015 | 11 Pages |
Abstract
Even though size exclusion chromatography (SEC) with post column addition of calcofluor (SEC-calcofluor) has been used for the determination of cereal β-glucan molar mass in foods for many years, there is a lack of systematic evaluation of the method. To address this issue a set of suitable β-glucan standards were generated by preparative SEC and their molar mass characteristics were determined by analytical multi-detection SEC (refractive index (RI), light scattering). Each standard was then analysed by SEC-calcofluor at three different labs. As a direct comparison, the analyses were repeated with a RI detector. For SEC-calcofluor accurate measurements of weight average molar mass (Mw) can be made for β-glucan populations within 10-500 Ã 103 g/mol. Above this molar mass threshold there is an increasing tendency for underestimation of Mw. Precipitation of some β-glucan-calcofluor complexes may have delayed their transport into the detector.
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Anne Rieder, Svein Halvor Knutsen, Ann-Sissel T. Ulset, Bjørn E. Christensen, Roger Andersson, Atte Mikkelson, Päivi Tuomainen, Ndegwa Maina, Simon Ballance,