Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7791069 | Carbohydrate Polymers | 2014 | 8 Pages |
Abstract
In this study, a novel flat-plate ultrasound technology was developed to stimulate polysaccharides production from Phellinus igniarius mycelial fermentation. Three-factor-three-level Box-Behnken design was used to optimize ultrasonic treatment time, duty cycle time and culture time for a high yield of P. igniarius polysaccharides (PIPS). Optimal conditions were found to be ultrasound treatment time 65Â min, duty cycle time 25Â s, and culture time 3.8Â d that gave a maximum PIPS yield of 1.8002Â g/L, which increased â¼22.64% compared with the control (without any ultrasound). PIPS mainly contained low-molecular weight (MW) polysaccharides (3.1Â kDa, 80%) composed of glucose, rhamnose and mannose in a molar ratio of 11.0:14.0:1.0. PIPS with higher carbohydrate and uronic acid contents exhibited strong antioxidant activities in vitro. Laser scanning confocal microscope (LSCM) observations suggested that ultrasound could change the morphology and structure of P. igniarius mycelium, and accelerate the transfer of nutrients and metabolites.
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Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Henan Zhang, Haile Ma, Wan Liu, Juanjuan Pei, Zhenbin Wang, Huiji Zhou, Jingkun Yan,