Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7791090 | Carbohydrate Polymers | 2014 | 10 Pages |
Abstract
Two pectic polysaccharides, 50WCP-II-I and 100WCP-II-I, were obtained from 50 and 100 °C water extracts of Codonopsis pilosula roots by ion exchange chromatography and gel filtration. The study of the sub-fractions obtained after pectinase degradation showed that the complement fixation activities of these pectins are expressed mainly by their ramified regions. The structure studies of native and sub-fractions showed the 50WCP-II-I is a pectic polysaccharide, with long homogalacturonan regions (some of the galacturonic acid units were methyl esterified), interrupted by one short rhamnogalacturonan I (RG-I) region. The side chains of the RG-I region are arabinogalactan type I (AG-I) and type II (AG-II) attached on position 4 of rhamnose. The 100WCP-II-I has two main ramified regions, one is galacturonan region with AG-I side chain on position 2 of GalA, and the other one is RG-I region with AG-II side chain on position 4 of Rha.
Keywords
LMWrhamnogalacturonan type Iarabinogalactan type IAG-IPABR3-Deoxy-d-manno-2-octulosonic acidCodonopsis pilosulaRG-IIRG-IXylRHAGlcAarabinogalactan type IIAG-IIHMWRhamnogalacturonan IEtOHArabinoseASEFucFucoseGlcKDOEthanolGalacturonic acidGlucuronic acidARArhamnoseFruaccelerated solvent extractionFructosegel filtrationMannoseManhomogalacturonanMolecular weighthigh molecular weightlow molecular weightPolysaccharidesIon exchange chromatographyIECGalGalAGalactoseXyloseGlucose
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Yuan-Feng Zou, Xing-Fu Chen, Karl Egil Malterud, Frode Rise, Hilde Barsett, Kari Tvete Inngjerdingen, Terje Einar Michaelsen, Berit Smestad Paulsen,