Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7791848 | Carbohydrate Polymers | 2014 | 9 Pages |
Abstract
YCP, an α-d-glucan with molecular weight of 2.4 Ã 103 kDa, was isolated from the mycelium of marine fungus Phoma herbarum YS4108. It interacted with TLR2 and TLR4 to induce B cells proliferation and activation. Here, a series of homogenous LMWYCPs (namely LMWYCP-1 to LMWYCP-6) were obtained by acid degradation. LMWYCP-3 and LMWYCP-4 were capable of inducing the proliferation of B cells dramatically. Further research involved TLR defunctionalization and competitive binding assay demonstrated that the immunomodulating activities of LMWYCP-4 was TLR4-dependent but TLR2-independent, while that mediated by LMWYCP-3 was neither TLR2- nor TLR4-dependent. Together with the structural information that LMWYCPs shared the similar structure features with YCP, we deduced that LMWYCP-4 may be the functional unit of YCP responsible for YCP/TLR4 interaction. This is the first time that the probable functional unit of glucan with the structure of α-d-(1-4)-linked residues in main chain and α-d-(1-6)-linked residues in branches is reported.
Related Topics
Physical Sciences and Engineering
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Authors
Rui Zhu, Xian Zhang, Wei Liu, Yan Zhou, Ran Ding, Wenbing Yao, Xiangdong Gao,