Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7792556 | Carbohydrate Polymers | 2014 | 7 Pages |
Abstract
This study reports on the purification and characterization of thermostable α-amylase (α-1-4 d-glucan glucanohydrolase EC 3.2.1.1) from a newly isolated Anoxybacillus flavithermus. A. flavithermus was used, which was isolated from hot water springs of Ãmer, Afyonkarahisar, Turkey. The gram-positive, spore-forming, motile, moderately thermophilic bacteria were found to be a strain of A. flavithermus analysed by 16S rRNA comparison. The optimal conditions for bacterial growth were determined to be at 20th h, 55 °C and pH 6.0. Maximum α-amylase activity was obtained at 55 °C at pH 7.0 after 24 h of incubation. Thermostable α-amylase from A. flavithermus was purified by 70% (NH4)2SO4 and ion-exchange chromatography (5.2-fold; 65.8% yield). The molecular weight of α-amylase was 60 kDa, as estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The α-amylase hydrolyzed soluble starch at 55 °C with Km: 0.005 mM and Vmax: 3.5 μmol minâ1.
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Physical Sciences and Engineering
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Authors
S. AgüloÄlu Fincan, B. Enez, S. Ãzdemir, F. Matpan Bekler,