Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7792564 | Carbohydrate Polymers | 2014 | 7 Pages |
Abstract
Poly(ethylene glycol)-conjugated N-(2-hydroxy) propyl-3-trimethyl ammonium chitosan chloride (PHTAC) derivatives were prepared by incorporating PEG molecules onto quaternized chitosan backbone. The copolymers were characterized by FTIR, 1H NMR and XRD. Agarose gel retardation assay indicated that PHTAC had good plasmid DNA (pDNA) binding capability and the particle sizes of PHTAC/pDNA complexes determined by DLS were about 200Â nm. Cytotoxicity assays in HeLa and 293T cells showed that PHTAC had low cytotoxicity. In vitro luciferase assay showed that PHTAC with PEGylation degree of 9% (PHTAC-1) had good transfection efficiency about 5.3-fold higher than quaternized chitosan, which was comparable with PEI (25Â kDa). These results suggest that PHTAC-1 is a promising candidate as an efficient nonviral gene vector.
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Authors
Xi Zhang, Juan Yao, Lihong Zhang, Jianguo Fang, Fengling Bian,