A comparison study on extraction of polysaccharides from Tricholoma matsutake by response surface methodology

The Tricholoma matsutake polysaccharides (TMP) extraction was optimized using counter-current probe ultrasonic extraction (CCPUE) by response surface methodology (RSM). The effect of three factors (liquid-solid ratio, extraction time and pulse duration) on the TMP yield was studied. The results showed that the optimal conditions were liquid-solid ratio (15), extraction time (61 min) and pulse duration (6.0 s). Under best conditions, the maximum TMP yield was 8.11 ± 0.35%, which was close with the predicted yield value (8.03%). The five methods, hot-water extraction (HWE), enzyme assisted extraction (EAE), ultrasonic clearer extraction (UCE), static probe ultrasonic extraction (SPUE), and CCPUE for extracting TMP by RSM were further compared. The results indicated that CCPUE had the largest yield of TMP with modest energy consumption.