Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7793010 | Carbohydrate Polymers | 2014 | 8 Pages |
Abstract
The enzyme-assisted extraction (EAE) of polysaccharides from the fruits of Hericium erinaceus was studied. In this study, response surface methodology and the Box-Behnken design based on single-factor and orthogonal experiments were applied to optimize the EAE conditions. The optimal extraction conditions were as follows: a pH of 5.71, a temperature of 52.03 °C and a time of 33.79 min. The optimal extraction conditions resulted in the highest H. erinaceus polysaccharides (HEP) yield, with a value 13.46 ± 0.37%, which represented an increase of 67.72% compared to hot water extraction (HWE). The polysaccharides were characterized by FT-IR, SEM, CD, AFM, and GC. The results showed that HEP was composed of mannose, glucose, xylose, and galactose in a molar ratio of 15.16:5.55:4.21:1. The functional groups of the H. erinaceus polysaccharides extracted by HWE and EAE were fundamentally identical but had apparent conformational changes.
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Authors
Yang Zhu, Qian Li, Guanghua Mao, Ye Zou, Weiwei Feng, Daheng Zheng, Wei Wang, Lulu Zhou, Tianxiu Zhang, Jun Yang, Liuqing Yang, Xiangyang Wu,