| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7793344 | Carbohydrate Polymers | 2012 | 9 Pages |
Abstract
⺠The use of derivative signals of the calcofluor/β-glucan complex emission spectra improved calibration. ⺠A low detection limit of 0.045 μg/mL (Clayton criterion) was achieved. ⺠β-glucan quantification in special arabinoxylan samples correlated well with an enzyme based assay. ⺠An in vitro model with differentiated Caco-2 cells revealed transport of cereal β-glucan over intact cell layers.
Keywords
PBSRMSECCalcofluorDMEMTEERFIADulbecco's modified Eagle MediumPCAFlow injection analysisPrincipal component analysisRoot Mean Square Error of CalibrationCereal β-glucanPhosphate buffered salineFluorescence spectroscopySEMtransepithelial electrical resistanceScanning electron microscopyMolecular weightWeight average molecular weightCell culture
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Anne Rieder, Svein H. Knutsen, Simon Ballance, Stine Grimmer, Diego Airado-RodrÃguez,