| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7794055 | Carbohydrate Research | 2015 | 6 Pages |
Abstract
- Four putative neuraminidases from a human gut bacterium were cloned and recombinantly expressed in active form.
- Functional characterisation revealed that the enzymes had similar temperature optima, but distinct metal requirements and pH optima.
- The enzymes showed different substrate specificities towards C5-modified sialic acid analogues.
- Simple expression and purification procedure make these enzymes potentially valuable for bioanalytical and industrial applications
Keywords
Related Topics
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Organic Chemistry
Authors
Kun Huang, Mao M. Wang, Anna Kulinich, Hong L. Yao, Hong Y. Ma, Juana E.R. MartÃnez, Xu C. Duan, Huan Chen, Zhi P. Cai, Sabine L. Flitsch, Li Liu, Josef Voglmeir,