Structural studies and biosynthetic aspects of the O-antigen polysaccharide from Escherichia coli O42

- The O-antigen polysaccharides of E. coli O28ac and O42 share the same trisaccharide-glycerol backbone.
- Dephosphorylation with aqueous 48% hydrogen fluoride resulted in an oligosaccharide, which was sequenced by MS/MS.
- The stereogenic center in the glycerol moiety is predicted to have the R configuration based on NMR and genetic data.