| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7794498 | Carbohydrate Research | 2014 | 7 Pages |
Abstract
- Oligosaccharides attached to the Asn residue of a peptide were produced in vitro.
- Membrane fractions of Pyrococcus furiosus cells were used.
- The structure was established by chemical, 2D NMR, and MS/MS analyses.
- The N-glycan is a novel heptasaccharide containing two branching xylose residues.
Keywords
HMBCGlcTFAHSQCABEETOCSYPyrococcus furiosusNOESYNOELLOEnzymatic preparationnuclear overhauser effectTrifluoroacetic acidLipid-linked oligosaccharideOstOligosaccharyltransferaseTamraNMRROESYNOE spectroscopyTotal correlation spectroscopycorrelation spectroscopyheteronuclear multiple-bond correlationCOSYgas–liquid chromatographyheteronuclear single quantum coherence
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Daisuke Fujinami, Masaki Matsumoto, Takuya Noguchi, Kenji Sonomoto, Daisuke Kohda,