| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7794740 | Carbohydrate Research | 2013 | 10 Pages |
Abstract
- We optimized the selectivity of DIFO-based reagents for examining intracellular glycans.
- We illustrated the potential non-specific labeling with difluorinated cyclooctynes.
- Biotin- and TAMRA-DIFO are highly reactive toward a reactive free peptidylcysteine.
- Biotin- and TAMRA-DIFO are almost inert to nonpeptidyl thiols (DTT, glutathione, etc.).
- Blocking of the free-thiols prior to SPAAC reaction restores its bioorthogonal nature.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Organic Chemistry
Authors
Eun J. Kim, Dong W. Kang, Hans F. Leucke, Michelle R. Bond, Salil Ghosh, Dona C. Love, Jong-Seog Ahn, Dae-Ook Kang, John A. Hanover,