Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12

Article ID	Journal	Published Year	Pages	File Type
7795020	Carbohydrate Research	2012	6 Pages	PDF

Abstract

âº Metabolic engineering of E. coli K-12 allows intracellular production of heparosan. âº The intracellular location of heparosan allows subsequent enzymatic modifications. âº Co-expression of K5-lyase allows production of heparosan oligosaccharides.

Keywords

E. coli K5 polysaccharide Heparosan Lyase Metabolic engineering Glycosaminoglycans

Related Topics

Physical Sciences and Engineering Chemistry Organic Chemistry

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Production of intracellular heparosan and derived oligosaccharides by lyase expression in metabolically engineered E. coli K-12

Authors

HélÃ¨ne Barreteau, Emeline Richard, Sophie Drouillard, Eric Samain, Bernard Priem,