Bioactive Ti-base biomaterial with sustained anti-bacterial response for endosseous applications

This paper aims to develop a bioactive biomaterial based on porous titanium coated with chitosan (Chi) hydrogel film (Chihf), which releases antibiotic, for tissue engineering of bone. The chosen antibiotic was Tetracycline (Tet); as a carrier, we used core-shell microcapsules of Bovine Serum Albumin (BSA) gel with k-carrageenan/chitosan polyelectrolite complex multilayer shell (ms). The structures of both Chihf and Tet-loaded microcapsules (Tet-ms) were characterized by: FTIR, XRD, SEM, CLSM, Fluorescence spectroscopy, and Fluorescence optical microscopy. The Tet release in pH 7.4 PBS is much slower from Tet-ms embedded in Chihf than simply from Tet-ms. The values of mechanical properties of Chi films reveal significant adhesiveness to the “skin model” film, a good premise for implant anchorage and osseointegration. The in vitro biological study of the film that releases the antibiotic indicates a good biocompatibility and the assessment of antibacterial activity shows a prolonged release of Tet, avoiding the biofilm formation and its adhesion.