Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7835286 | Applied Surface Science | 2018 | 10 Pages |
Abstract
In this study, poly(vinyl chloride) (PVC) was electrospun into fibrous membranes and then reacted with NaN3 to generate azido-terminated PVC fibrous membranes. A propargyl-terminated poly(N-isopropylacrylamide) (PNIPAAm) was also synthesized and then grafted, through click reactions, onto the azido-terminated PVC fiber surface. Protrusion-, scale-, and joint-like structures of the PNIPAAm grafts on the PVC fibers were formed upon increasing the molecular weight of the PNIPAAm grafts. The PNIPAAm-grafted PVC fibrous mats exhibited completely wetted surfaces at 25â¯Â°C because of their high roughness. The static water contact angle of the PNIPAAm-grafted PVC fibrous mats reached 140° when the temperature was increased to 45â¯Â°C. This thermoresponsive behavior was significantly greater than that of the PNIPAAm grafted on a flat surface. Temperature-responsive membranes were constructed having a pore size of 1.38â¯Î¼m and applied as protein valves to block and release an antibody (fluorescein-conjugated AffiniPure goat anti-rabbit IgG). At 25â¯Â°C, the collection efficiency remained at 94% for antibody concentrations up to 60â¯ng/L. As the temperature increased to 45â¯Â°C, the collection efficiency decreased abruptly, to 4%, when the antibody concentration was greater than 20â¯ng/L. Accordingly, this system of PNIPAAm-grafted PVC fibers functioned as a protein valve allowing the capture and concentration of proteins.
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Physical and Theoretical Chemistry
Authors
Jian-Wei Guo, Zhen-Yu Lin, Chi-Jung Chang, Chien-Hsing Lu, Jem-Kun Chen,