Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7865605 | Materials Science and Engineering: C | 2018 | 10 Pages |
Abstract
Porous scaffolds fabricated with nano-hydroxyapatite (nHA) and chitosan (CTS), are widely used in bone tissue engineering (BTE). However, cell adhesion is relatively poor in nHA/CTS scaffolds, which also do not provide an ideal three-dimensional environment for seed cells. These deficiencies limit the applicability of these BTE scaffolds to repair bone defects. To address these challenges, we designed a composite scaffold that combines nHA/CTS with self-assembling peptide (SAP), a material which is similar to the extracellular matrix. We found that SAP/nHA/CTS scaffolds both increased the adhesion of bone mesenchymal stem cells (BMSCs) and enhanced the mechanical properties of the scaffold. This composite scaffold was then used to repair a femoral condylar bone defect in a mouse model. Healing and mineralization was demonstrated after 12â¯weeks using H&E staining, microcomputerized tomography, and bone mineral density tests. To our knowledge, this is the first report that SAP/nHA/CTS scaffolds can increase cell adhesion and promote the reconstruction of femoral condylar bone defects. Moreover, this study indicates that BTE using a SAP/nHA/CTS scaffold may be a novel prospective strategy for healing extensive bone defects.
Related Topics
Physical Sciences and Engineering
Materials Science
Biomaterials
Authors
Zhiming Zhang, Guofeng Wu, Yanlin Cao, Chun Liu, Yanglei Jin, Yihan Wang, Lianjun Yang, Jiasong Guo, Lixin Zhu,