Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7865679 | Materials Science and Engineering: C | 2018 | 24 Pages |
Abstract
In this study, a Y-shaped amphiphilic block copolymer consisting of hydrophilic poly(ethylene glycol) (mPEG) and two poly(ε-caprolactone) (PCL) as the hydrophobic arms with a ketal linker was synthesized (mPEG-Ketal-(PCL)2). The structure of the copolymer with different compositions was characterized by 1H NMR, gel permeation chromatography (GPC) and differential scanning calorimetry (DSC). The amphiphilic property endows the copolymer with the ability to be self-assembled into micelles for encapsulating anticancer drug doxorubicin (DOX), and the effect of copolymer with different PCL length on drug loading properties were tested. The morphology, size, pH responsiveness, drug release profile and in vitro anticancer activity of the DOX loaded micelles were also studied. In vitro drug release studies showed that over 50% of the DOX was released at pHâ¯5.0 in 24â¯h because of hydrolysis of ketal linker in the copolymers. The confocal laser microscopy and flow cytometry experiments showed that the DOX-loaded micelles could be effectively internalized by Hela cells, the ketal bond in the backbone was broken in acid endo/lysosomal compartments and triggered the fast release of DOX. The in vitro antitumor efficacy of the DOX loaded mPEG-Ketal-(PCL)2 micelles was better than that of non-pH sensitive ones. In vivo studies showed that the mPEG-Ketal-(PCL)2 micelles enhanced the DOX blood circulation time and had good tumor-targeting efficiency.
Related Topics
Physical Sciences and Engineering
Materials Science
Biomaterials
Authors
Yujiao Zhang, Yujie Lu, Ming Cao, Peng Chen, Bin Yang, Jibin Miao, Ru Xia, Jiasheng Qian,