Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7873699 | Synthetic Metals | 2018 | 9 Pages |
Abstract
In the present work, a platform of Poly (3, 4-ethylenedioxythiophene) (PEDOT) and graphene oxide (GO) composite decorated with spherical gold nanoparticles (AuNPs) has been developed for rapid electrochemical detection of aflatoxin B1 (AFB1). Electrochemical deposition of EDOT onto glassy carbon electrode (GCE) keeping GO as dopant followed by introduction of AuNPs has been achieved resulting in nanohybrid AuNPs/PEDOT-GO electrode. The antibody anti-aflatoxin B1 (anti-AFB1) has been further covalently immobilized onto the surface of AuNPs/PEDOT-GO using EDC/NHS coupling. The morphological and surface characteristics have been studied using Field Emission Scanning Microscopy (FESEM) and contact angle measurements. Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) studies have been carried out to investigate the electro-catalytic behavior of the modified electrodes. The heterogeneous rate constant (ks) and transfer coefficient (α) have been determined by using Laviron's method. The proposed immunosensor exhibits a very high sensitivity of 0.989â¯Î¼Aâ¯ngâ¯mLâ1 and 0.397â¯Î¼Aâ¯ngâ¯mLâ1 within two linear range of 0.5-20â¯ngâ¯mLâ1 and 20-60â¯ngâ¯mLâ1, respectively.
Related Topics
Physical Sciences and Engineering
Materials Science
Biomaterials
Authors
Arati Sharma, A. Kumar, Raju Khan,