| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7931217 | Optics Communications | 2014 | 6 Pages |
Abstract
A new method based on the subtraction of images is proposed to enhance the resolution and contrast in CW-STED microscopy. It is realized by the subtraction of a confocal image from a STED image. Detailed theoretical analyses and a series of simulation tests are performed. The validity of this subtraction method in practice is demonstrated by experiments on both fluorescent nanoparticles and microtubule networks in which a better resolution and contrast than the STED image is achieved. Subtractive parameters are discussed in detail and a way of choosing the optimal value is proposed. Compared with other resolution enhancement concepts, this method is much simpler and free from using high intensity lasers.
Keywords
Related Topics
Physical Sciences and Engineering
Materials Science
Electronic, Optical and Magnetic Materials
Authors
Yue Fang, Yifan Wang, Cuifang Kuang, Xu Liu,