Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8015153 | Materials Letters | 2018 | 12 Pages |
Abstract
In this study, polyethersulfone (PES) paper embedded with a polymethyl methacrylate (PMMA) platform was developed for simultaneous DNA amplification and colorimetric detection. DNA was amplified using loop-mediated isothermal amplification (LAMP) in isothermal conditions in the presence of the common magnesium-dependent colorimetric indicator, eriochrome black T (EBT), to detect DNA. Here, the DNA of Staphylococcus aureus was used as the DNA template. The developed device was successfully used to simultaneously amplify and detect the DNA by the naked eye within 30â¯min with a detection limit of 1â¯fg/µL.
Keywords
Related Topics
Physical Sciences and Engineering
Materials Science
Nanotechnology
Authors
Jae-Heon Kim, In Sang Yoo, Jeong Ho An, Sanghyo Kim,