Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8037691 | Ultramicroscopy | 2018 | 24 Pages |
Abstract
A new SEM-based in-line electron holography microscope has been under development. The microscope utilizes conventional SEM and BF-STEM functionality to allow for rapid searching of the specimen of interest, seamless interchange between SEM, BF-STEM and holographic imaging modes, and makes use of coherent low-energy in-line electron holography to obtain low-dose, high-contrast images of light element materials. We report here an overview of the instrumentation and first experimental results on gold nano-particles and carbon nano-fibers for system performance tests. Reconstructed images obtained from the holographic imaging mode of the new microscope show substantial image contrast and resolution compared to those acquired by SEM and BF-STEM modes, demonstrating the feasibility of high-contrast imaging via low-energy in-line electron holography. The prospect of utilizing the new microscope to image purified biological specimens at the individual particle level is discussed and electron optical issues and challenges to further improve resolution and contrast are considered.
Related Topics
Physical Sciences and Engineering
Materials Science
Nanotechnology
Authors
Hidehito Adaniya, Martin Cheung, Cathal Cassidy, Masao Yamashita, Tsumoru Shintake,