Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8056889 | Acta Astronautica | 2014 | 8 Pages |
Abstract
The aim of the current study is to investigate effects of simulated microgravity on the cell cycle of endothelial cells. We analyze changes in the cell cycle after exposure of endothelial-like EA.hy 926 cells to simulated microgravity using a Desktop random positioning machine (RPM). Cell cycle profiles determined by flow cytometry show, that the percentage of the cells in the G0/G1 phase after 24 and 96Â h of RPM-simulated microgravity is significantly increased as compared to the control group. However, no significant difference is observed after 120Â h of RPM-simulated microgravity. In regard to S phase, the percentage of cells is significantly decreased after 24 and 96Â h of RPM, respectively; whereas 120Â h later, the number of S-phase cells is comparable to the control group. Thus, we show that simulated microgravity inhibits cell cycle progression of human EA.hy 926 cells from the G0/G1 phase to the S phase. We observe an effect of a hibernation-like state, when the growth of the cells in the RPM group slows down, but does not stop. Our results further show that simulated microgravity can affect adhesion of endothelial cells, and alpha-tubulin expression, as most cells begin to detach from the surface of OptiCell unit after 24Â h, form aggregates after 48Â h, and exhibit accumulation of alpha-tubulin around the nucleus after 48Â h of exposure to simulated microgravity conditions. Our results demonstrate a chance in the cell cycle in a low gravitational field.
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Physical Sciences and Engineering
Engineering
Aerospace Engineering
Authors
Alisa A. Sokolovskaya, Tatiana I. Ignashkova, Anna V. Bochenkova, Aleksey A. Moskovtsev, Victor M. Baranov, Aslan A. Kubatiev,