Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8085650 | Algal Research | 2018 | 9 Pages |
Abstract
For this, a new GUS reporter vector pBINâ¯+â¯TetRâ¯+â¯TetO was developed, followed by transformation (Agrobacterium tumefaciens), screening and characterization of the transgenic C. vulgaris. In the screening study, strain number 18 showed the highest fluorescence intensity (16,988â¯Â±â¯1168). The GUS enzyme was found to be stable for >8â¯h for intact cell and lysed cell studies. The optimum concentration of Triton X-100 to release the product (4-Methylumbelliferone) into buffer was 0.1% and the fluorescence intensity was 28,397â¯Â±â¯787. The values of Km and Vmax of the recombinant GUS for lysed cells were 0.1304â¯Â±â¯0.0101â¯mM and 0.35â¯Â±â¯0.004â¯pmolâ¯4-MU/min/ml of crude cell lysate respectively.
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Renewable Energy, Sustainability and the Environment
Authors
Shreyas Yedahalli, Lars Rehmann, Amarjeet Bassi,